منابع مشابه
Establishment, Culture and Freezing of Human and Mouse Embryonic Stem Cells: a Protocol Guide
Studies of the biology of human embryonic stem cells (hES cells) have developed rapidly over the past nine years since the first reports of their derivation. They clearly offer enormous potential, not only for regenerative medicine, but also for drug discovery and toxicology, human developmental biology and cancer research. Realizing these potentials a better understanding of the fundamental as...
متن کاملEffects of Mouse Strain on Establishment of Embryonic Stem Cell Lines
Purpose: Embryonic stem (ES) cells are derived from the inner cell mass of blastocysts with self-renewal and pluripotency characteristics. These cells have potential for studies of in vitro differentiation, gene function, etc. This study was, therefore, initiated to establish new ES lines and evaluate the effects of strain on ES cell production. Materials and Methods: 3-5 day blastocysts were ...
متن کاملCytokinesis and the establishment of early embryonic cell polarity.
Cleavage divisions in many animals form a blastula made up of a simple polarized epithelium. This simple embryonic epithelium possesses an apical surface covered with microvilli and primary cilia separated from the basolateral surfaces by cell-cell junctions. The apical membrane proteins and lipids differ from those of the basolateral on these embryonic epithelial cells, as is found in adult ep...
متن کاملEstablishment of Embryonic Stem Cell-like Cells in Pigs
We generated unique porcine embryonic stem cell-like cells (pESLCs) by using inhibitors of GSK3β and MEK1. The pESLCs colonies are dome-shape morphology and show the expression of undifferentiated markers including OCT4 and NANOG. Moreover, the self-renewal capacity and morphology of the cell lines are LIFdependent, consistent with the LIF receptors expression and STAT3 phosphorylation, and con...
متن کاملEstablishment of Tsc2‑deficient rat embryonic stem cells.
Tuberous sclerosis complex (TSC) is an autosomal dominant disorder caused by TSC1 or TSC2 mutations. TSC causes the development of tumors in various organs such as the brain, skin, kidney, lung, and heart. The protein complex TSC1/2 has been reported to have an inhibitory function on mammalian target of rapamycin complex 1 (mTORC1). Treatment with mammalian target of rapamycin (mTOR) inhibitors...
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ژورنال
عنوان ژورنال: Plant Signaling & Behavior
سال: 2013
ISSN: 1559-2324
DOI: 10.4161/psb.27491